15th October 2019
A new research paper based upon International collaboration between Takashi Toda’s group and the Francis Crick Institute (London, UK) was published in International Journal of Molecular Sciences online. This article belongs to the Special Issue “Mechanism of Chromosome Segregation in Eukaryotes”.
This work is partly supported by “Program For Fostering Globally Talented Research” JSPS grant (S2902/JPMX05S2900002). Dr Corinne Pinder stayed in Takashi Toda’s laboratory in 2018 (January-May) to carry out this collaborative work.
Masashi Yukawa, Tomoki Kawakami, Corinne Pinder and Takashi Toda
Two XMAP215/TOG Microtubule Polymerases, Alp14 and Dis1, Play Non-Exchangeable, Distinct Roles in Microtubule Organisation in Fission Yeast
International Journal of Molecular Sciences 2019 : 20(20), 5108
3rd October 2019
Takashi Toda and Masaru Ueno met Drs Miguel Godinho and Rita Ferreira in Hiroshima. They visited Japan to attend the 78th Annual Meeting of the Japanese Cancer Association (第７８回日本癌学会学術総会) held in Kyoto. Miguel used to work in the Cancer Research UK, in which Takashi ran the lab. Masaru know Miguel very well as they both have been working on telomeres in fission yeast. Miguel has been working as a lab head/principal investigator in the Institute for Research on Cancer and Aging, Nice in France.
1st October 2019
A new research paper based upon International collaboration between Takashi Toda’s group and the Francis Crick Institute (London, UK) was published in a new issue of Journal of Cell Science. This work is partly supported by “Program For Fostering Globally Talented Research” JSPS grant (S2902). The first author Dr Corinne Pinder stayed in Takashi Toda’s laboratory in 2018 (January-May) to carry out this research project.
Corinne L. Pinder, Yuzy Matsuo, Sebastian P. Maurer and Takashi Toda
Kinesin-8 and Dis1/TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation in fission yeast
Journal of Cell Science 2019 : 132, 1-15.
20th September 2019
Takashi Toda attended the 24th board member meeting of Federation of Microbiological Societies of Japan held at Science Council of Japan in Tokyo.
11th September 2019
Takashi Toda gave an oral talk in the 91st annual meeting of the Genetic Society of Japan held at Fukui University.
The title of his talk was ｀Molecular genetic study of the microtubule cytoskeleton （酵母を用いた細胞骨格の分子遺伝学的研究：微小管を中心として）.
4th-6th September 2019
4 lab members (Takashi Toda, Masashi Yukawa, Mitsuki Ohishi and Yasuhiro Teratani) attended 52nd annual meeting of Yeast Genetic Society of Japan held in Shizuoka City Shimizu Cultural Hall MARINART and presented their works.
Yasuhiro Teratani: Novel functions of fission yeast Kinesin-5/Cut7 during cell cycle M phase（分裂酵母5型キネシンCut7の細胞周期M期における新規機能）
Mitsuki Ohishi: Functional analysis of a novel RNA-binding protein Nrp1 in fission yeast（分裂酵母の新規RNA 結合タンパク質Nrp1の機能解析）
Masashi Yukawa: Dual roles of fission yeast Kinesin-6/Klp9 in the spindle formation and elongation during anaphase B（M期後期の紡錘体形成・伸長における分裂酵母6型キネシンKlp9の役割）
Takashi Toda: Crosstalk between factors involved in microtubule polymerization and depolymerization to ensure proper spindle length（正確なスピンドル長を規定する微小管重合・脱重合因子のクロストーク）
Lower photo: from the left, Yoshikazu Ohya (Former President of YGSJ, The University of Tokyo), Takashi Toda, Yasuhiro Teratani, Mitsuki Ohishi, Masashi Yukawa
19th August 2019
A new research paper based upon International collaboration between Takashi Toda’s group and the Francis Crick Institute (London, UK) was published in the Journal of Cell Science online. This work is partly supported by “Program For Fostering Globally Talented Research” JSPS grant (S2902). The first author Dr Corinne Pinder stayed in Takashi Toda’s laboratory in 2018 (January-May) to carry out this research project.
Corinne L. Pinder, Yuzy Matsuo, Sebastian P. Maurer and Takashi Toda
Kinesin-8 and TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation
High-fidelity chromosome segregation relies on proper microtubule regulation. Kinesin-8 has been shown to destabilise microtubules to reduce metaphase spindle length and chromosome movements in multiple species. XMAP215/chTOG polymerases catalyse microtubule growth for spindle assembly, elongation and kinetochore-microtubule attachment. Understanding of their biochemical activity has advanced but little work directly addresses the functionality and interplay of these conserved factors. We utilised the synthetic lethality of fission yeast kinesin-8 (Klp5-Klp6) and XMAP215/chTOG (Dis1) to study their individual and overlapping roles. We found that the non-motor kinesin-8 tailbox is essential for mitotic function; mutation compromises plus-end-directed processivity. Klp5-Klp6 induces catastrophes to control microtubule length and surprisingly, Dis1 collaborates with kinesin-8 to slow spindle elongation. Together, they enforce a maximum spindle length for a viable metaphase-anaphase transition and limit elongation during anaphase A to prevent lagging chromatids. Our work provides mechanistic insight into how kinesin-8 negatively regulates microtubules and how this functionally overlaps with Dis1 and highlights the importance of spindle length control in mitosis.
Journal of Cell Science 2019 : jcs.232306 doi: 10.1242/jcs.232306
Publication of this work was announced in the Web sites of Hiroshima University at https://www.hiroshima-u.ac.jp/ilife/research/biotechnology/topics (Graduate School of Integrated Sciences for Life) and https://www.hiroshima-u.ac.jp/adsm/news/52942 (AdSM).
9th August 2019
Nimisha Krishnan jointed our lab as an short-term international student. She is a master course student belonging to the Indian Institute of Science Education and Research at Mohali. She will conduct her master course study with us for 4 months.
8th August 2019
Toda/Yukawa group’s research was in the August issue of HU-plus, a biannual magazine published by public relations section of Hiroshima University.
5th August 2019
An undergraduate student from Harvard Unviersity, Hayato Shiotsu, has completed a 9 week summer replacement and left the lab. Well done, Hayato.
1st August 2019
Our new paper has been featured on the latest issue of the “HIROSHIMA UNIVERSITY UPDATE Vol.8”, published in July 2019. This University Magazine is published three times a year.
13th-18th July 2019
Takashi Toda attended the 10th international fission yeast meeting held in Barcelona, Spain and discussed research projects with Sir Paul Nurse and Dr Kazunori Kume. In the meeting, he gave a special lecture entitled “Pathways leading to mitotic spindle assembly: conventional and new views” as a keynote speaker and also did a poster presentation entitled “Interplay between two mitotic kinesins and the microtubule crosslinker drives spindle elongation during anaphase B”.
8th July 2019
Dr Katsura Asano, professor at Kansas State University (U.S.A.), visited Hiroshima University and discussed international collaborations with Takashi Toda.
24th June 2019
Takashi Toda attended the joint annual meeting of 71st Japanese Society for Cell Biology and 19th Protein Science Society of Japan, held at Kobe International Conference Center, Kobe.
He conducted both oral and poster presentations.
“Kinesin 6 Klp9 promotes microtubule elongation during anaphase B through motor-dependent and –independent manners”
Takashi Toda, Yasuhiro Teratani, Corinne Pinder, Ken-ya Furuta and Masashi Yukawa
(登田 隆, 寺谷康宏, Corinne Pinder, 古田健也、湯川格史)
21st June 2019
Dr Shoji Hata (Zentrum für Molekulare Biologie der Universit.t Heidelberg) gave a talk as a speaker of the 40th HiHA seminar.
The title of his talk was “Timely assembly of the mitotic spindle for accurate chromosome segregation”.
14th June 2019
Naoaki Kurisawa, our collaborator in Keio University (formerly a master course student in Professor Ken-ichi Kimura’s laboratory at Iwate University) was awarded the best poster prize at the 18th meeting on the exploration of novel materials (JANPBR). The title of his presentation was “Exploring novel HSET inhibitors from natural products using fission yeast cells overexpressing HSET” (HSET 過剰発現分裂酵母株を用いた新規天然物由来 HSET 阻害剤の探索).
4th June 2019
Hayato Shiotsu (塩津颯人) joined our group from Harvard University as a summer replacement student. He came to Hiroshima University through a student exchange programme between the two universities and is supported by Hiroshima University and RIJS (Edwin O. Reischauer Institute of Japanese Studies) Henry Rosovsky Summer Research Travel Grant. He will stay with us for two months.