Previous lab news 2017

18th and 19th December

Takashi Toda visited the Francis Crick Institute and discuss research collaboration with Paul Nurse’ group. Corinne Pinder, to whom Takashi Toda has been a supervisor for her PhD course successfully passed viva and will be awarded PhD.

Photos, Takashi Toda and Corinne Pinder
Takashi Toda, Carolyn Moores (Professor at Birkbeck College, University of London) and Juan Mata (Reader in University of Cambridge)
Corinne Pinder、Carolyn Moores and Juan Mata.
Drs Mata and Moores are examiners for Corinne Pinder’s thesis/viva.

1st December

A new paper derived from international collaboration between Hiroshima University-HiHA (Takashi Toda/Masashi Yukawa’s group and Kazunori KUme) and University of California, San Diego (Ngang Hoek Tang) was published in the 1st December issue of Molecular Biology of the Cell.

Authors: Masashi Yukawa, Tomoki Kawakami, Masaki Okazaki, Kazunori Kume, Ngang Heok Tang and Takashi Toda
Title: A microtubule polymerase cooperates with the Kinesin-6 motor and a microtubule crosslinker to promote bipolar spindle assembly in the absence of Kinesin-5 and Kinesin-14 in fission yeast
Journal: Molecular Biology of the Cell, vol. 28, no. 25, 3647-3659 (2017)

Abstract

24th November

Mitsuki Ohishi, Yasuhiro Teratani and Masashi Yukawa attended the 35th annual meeting of the YWS held at the Kagawa Prefectural Rexxam Hall in Takamatsu.
Mitsuki and Yusuhiro had an oral and poster presentation in the meeting.

Their titles were:

A genetic screen for suppressor mutants restoring the lethality caused by overexpression of Kinesin-14: Mitsuki
（分裂酵母14型キネシン過剰発現による致死性を抑圧する変異体の探索：大石）

Isolation and analysis of novel temperature-sensitive alleles of the fission yeast Kinesin-5/cut7 by PCR-mediated mutagenesis: Yasuhiro
（PCR法を用いた分裂酵母5型キネシンcut7新規変異体の分離と解析：寺谷）

11th October

A new paper based on international collaboration between Hiroshima University-HiHA (Takashi Toda/Masashi Yukawa’s group and Kazunori KUme) and University of California, San Diego (Ngang Hoek Tang) was published online in the web site of Molecular Biology of the Cell.

https://www.ncbi.nlm.nih.gov/pubmed/29021344

Title: A microtubule polymerase cooperates with the Kinesin-6 motor and a microtubule crosslinker to promote bipolar spindle assembly in the absence of Kinesin-5 and Kinesin-14 in fission yeast
Authors: Masashi Yukawa, Tomoki Kawakami, Masaki Okazaki, Kazunori Kume, Ngang Heok Tang and Takashi Toda

日本語

27th September

Professor Hirotada Mori (Nara Institute of Science and Technology) gave a talk at Department of Science hosted by Professor Katsunori Suzuki. The title was “Systems biology in E. coli utilising comprehensive resources: What could we learn from global analysis in E. coli?” (網羅的リソースを活用した大腸菌システムズ生物学：大腸菌を用いた網羅解析から何が得られるのか). Dr Hirotada Mori is a friend of Dr Toda’s, since they worked at Kyoto University as assistant professors nearly 30 years ago. Dr Suzuki used to work on yeast when he was a student of Professor Yanagishima in Nagoya University.

Photo
The photo shown is Professor Hirotada Mori (centre) and Professor Katsunori Suzuki (left).

11th-13th September

Tomoki Kawakami, Yusuke Yamada, Masashi Yukawa and Takashi Toda attended the 50th annual meeting of the YGSJ held at the Yayoi-Kodo conference hall, the University of Tokyo. Masashi, Tomoki and Yusuke had an oral presentation in the meeting.

Their titles were:

A novel mechanism of bipolar spindle assembly through the Alp7/TACC-Alp14/TOG, microtubule polymerase protein complex in fission yeast: Tomoki
（分裂酵母の微小管ポリメラーゼ複合体 Alp7/TACC- Alp14/TOG による新たな双極性紡錘体形成機構：河上）

Suppressor analysis of temperature-sensitive mutations of the Kinesin-5/Cut7 in fission yeast: Yusuke
（分裂酵母 Kinesin- 5/Cut7 の温度感受性変異を抑圧する変異株の解析：山田）

Investigation of a Kinesin-5 independent pathway leading to bipolar spindle formation: Masashi
（5型キネシンに依存しない新規紡錘体形成経路の探索：湯川）

20th and 21st July

Dr Masato Kanemaki, Professor at the National Institute of Genetics, gave a special lecture (遺伝子編集技術発展の歴史，背景，現状，未来，またその社会的意義について, Lecture on gene editing in terms of its history, updates and impact on the society to graduate school students of Department of Molecular Biotechnology, AdSMGraduate School of Advanced Sciences of Matter. He also gave a talk in the 19th HiHA seminar. His title was 「オーキシンデグロン技術が切り開くヒト細胞における新たな染色体生物学」(A new frontier of chromosome biology in human cells implemented by Auxin-Degron technology)

11th May
A new paper was published from Dr Takashi Toda’s group.

Purification and characterisation of the fission yeast Ndc80 complex
Protein Expression and Purification, 135 (2017) 61-69 DOI: 10.1016/j.pep.2017.05.002 (
http://www.sciencedirect.com/science/article/pii/S104659281730075X)).

This work was performed under international collaboration of four laboratories; Takashi Toda (Hiroshima University), Thomas Surrey (The Francis Crick Institute, London, UK), Martin R Singleton (The Francis Crick Institute, London, UK) and Sebastian Maurer (Universitat Pompeu Fabra, Barcelona, Spain) .

14th-19th May 2017

Takashi Toda and Masashi Yukawa attended the 9th international Fission Yeast Meeting held at the Banff Centre in Canada. Masashi gave an oral talk (Assembly of Mitotic Bipolar Spindle in the Absence of Kinesin-5 Cut7) and Takashi gave a poster presentation (Towards the in vitro Reconstitution of the Kinetochore-Microtubule Interface).

In addition, Corinne Pinder, a graduate student in the Francis Crick Institute, London UK, whom Dr Toda has been remotely supervising from Hiroshima University and who currently belongs to Dr Paul Nurse’s group, also gave an oral prevention (Exploring the Mitotic Roles of Kinesin-8 in Fission Yeast).

17th April 2017

Molecular mechanisms of genome stability control in cell proliferation: lessons from yeast

Takashi Toda gave a talk at Hotel Granvia. The title was “Molecular mechanisms of genome stability control in cell proliferation: lessons from yeast”. This seminar was hosted by Professor Kazuaki Chayama (the Division head of Gastroenterology, Graduate School of Biomedical and Health Science, Hiroshima University) and sponsored by Bristol-Myers Squibb K.K. as Hiroshima City B-type Hepatitis seminar series.

Takashi Toda and Gota Goshima (Professor at Nagoya University)